Acidithiobacillus ferrooxidans ATCC 23270

View alignment of region with nucmer

Design primers within region using Primer3Plus

View region in Artemis

Retrieve AFE_0002 nucleotide sequence in FASTA format

Retrieve AFE_0002 protein sequence in FASTA format

Retrieve AFE_0002 nucleotide sequence with 10 50 100 200 400 600 800 1000 2000 bp. flanking sequence
Include translation of coding sequence
Include complementary strand.

Run NCBI BLASTP PSI-BLAST against the Non-redundant protein sequences (nr) SwissProt Protein Data Bank (PDB) database using AFE_0002 protein sequence
(optional - restrict search to taxonomic group: root cellular organisms Bacteria Proteobacteria Gammaproteobacteria Acidithiobacillales Acidithiobacillaceae Acidithiobacillus Acidithiobacillus ferrooxidans )

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