Streptococcus pneumoniae TIGR4

View alignment of region with nucmer

Design primers within region using Primer3Plus

View region in Artemis

Retrieve SP_1225 nucleotide sequence in FASTA format

Retrieve SP_1225 protein sequence in FASTA format

Retrieve SP_1225 nucleotide sequence with 10 50 100 200 400 600 800 1000 2000 bp. flanking sequence
Include translation of coding sequence
Include complementary strand.

Run NCBI BLASTP PSI-BLAST against the Non-redundant protein sequences (nr) SwissProt Protein Data Bank (PDB) database using SP_1225 protein sequence
(optional - restrict search to taxonomic group: root cellular organisms Bacteria Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus Streptococcus pneumoniae )

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